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Amplicon+High-throughput qPCR chip

Amplicon+High-throughput qPCR chip


Product Introduction

16S rDNA gene is the most commonly used molecular marker for studying the evolution and classification of prokaryotic microorganisms. It is widely used in microbial ecological research. High-throughput qPCR (HT-qPCR) chips are quantitatively more accurate compare with the sequencing methods, and are widely used for microbial and functional diversity analyses in various ecosystems.

Chips

Number of genes 

(Including 16S rRNA)

Sample processing capacity per chip

(Including 3 technical replicates)

Antibiotic resistance gene chip(384P)

384

4

Antibiotic resistance gene chip(144P)

144

12

Carbon, nitrogen, phosphorus, sulfur functional gene chip

72

24

Arsenic functional gene chip

80

21

Heavy metal resistance gene chip

96

18

Virulence factor chip

120

14

 

Based on the 16S rRNA and high-throughput qPCR results, the differences in microbial communities and functional genes can be revealed. Further correlation analyses would help exploring their close associations. 

Analysis pipeline

  • Through the analysis of the flora and functional gene level, mutual verification;
  • At the same time to achieve from the "cause" and "effect" two aspects to explore biological problems;
  • This paper expounds the association mechanism between flora and functional genes to more comprehensively analyze the species and functional regulation mechanism in biological processes.

Analysis process

Flowchart of microbial diversity and High-Throughput qPCR chip joint analysis

Results demonstration

db-RDA Analysis

Structural Equation Model

VPA Analysis

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