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Metatranscriptome

Metatranscriptome


Product Introduction

Macro transcriptome sequencing is based on the whole microbial community in a specific environment as the research object, which gets rid of the technical limitations of difficult isolation and culture of microorganisms, and only needs to extract the total RNA of all microorganisms from the sample for sequencing.
Macrotranscriptome sequencing uses the Novaseq second-generation high-throughput sequencing platform to comprehensively sequence RNA of microbial communities in samples. After analysis of assembly, gene prediction, gene annotation and abundance calculation, genetic diversity and molecular ecological information such as species composition, functional gene transcription regulation and metabolic pathways in samples are excavated.
 

Product Features

  • Microbial communities can be identified at the transcriptional level to study community characteristics;
  • Identification of target pathogens;
  • Excavation of active microorganisms and their functions;
  • Focus on key genes and explore gene function and metabolic pathways.

Technical parameters

Products

sequencing platform

sequencing strategy

macro transcription group

New PE150

10 G raw data

Experimental process

Total RNA Extraction

RNA Quality Inspection

mRNA Purification and Fragmentation

double-stranded cDNA synthesis

End Repair Plus A and Fittings

Degradation of the second strand of cDNA

Fragment selection and PCR enrichment

Library Quality Inspection

on-board sequencing

Analysis process

 

Analysis results display

ternary phase diagram

Gene Number Petal Chart

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Magi Gene

Magi Kefu

Contact Us

Web:www.magigene.com

Address: Floor 13, Block B, Building 7, Phase 3, International Innovation Valley, Shenzhen

Address: 28th Floor, Building A, Guanzhou Life Science Center, Guangzhou International BioIsland

Address: 4F, Building 3, Phase 2, Guangzhou International Biological Island

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