The Magigene customized DNA microarray service offers DNA-DNA hybridization based microarray development, which can be used for high-throughput functional genes or strain-specific microbial identifications in complex samples. We are committed to promoting the application of DNA microarray in fields including scientific research and environmental monitoring.
The DNA microarray is a tool used to determine phylogenetic or functional diversity of microorganisms from a particular sample. The chip consists of a small piece of glass on which it contains millions of short, synthetic, single-stranded DNA sequences known as probes. The fluorescent-labeled DNA to be tested is hybridized with the probes on the microarray, and then the hybridization signal is detected to achieve rapid, accurate and efficient detection for the presence of genes or microorganisms of interests.
A highly specialized DNA microarray for high-throughput and quantitative detection of cyanobacteria at strain level in various environments. CyanoChip is designed with probes based on approximately 1,300 complete genomes of cyanobacteria. Each microarray contains 8 sub-matrices with 60,000 probes each, allowing parallel measurements of these cyanobacterial strains in a maximum of 16 samples such as water bodies and soil crusts.
- Rapid: Results can be achieved within 2 days
- .High sensitivity: ng-level of genomes can be detected.
- High specificity: avoid PCR amplification, minimum data distortion and high reproducibility.
- High throughput: maximum 16 sample processing capacity and millions of probes targeting the cyanobacteria of interests.
|The customer fills in and submits the customized chip research and development requirements form.
|Feasibility Assessment Confirmation
|Entrusted R & D service contract
|R & D of customized chip
|Customized Service Delivery
The process of customized services
Requirement submission by customer -- Feasibility assessment -- R&D contract confirmation – Microarray design and tests -- Reliability Verification and delivery
Microarray development process
The main steps of microarray development include: database construction of genomes/functional genes, probe design and validation, microarray layout setting, probes and microarray syntheses, hybridization tests, validation and delivery.
- Recommended species limitation for identification: 50~2000. The actual number is flexible depends on the number of qualified probes available for detection.
- Large number of pure cultures for microarray hybridization and validation is preferred, normally more than 10% of the target species.
Information to be filled in when ordering products:
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