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Metavirome

Metavirome


Product Introduction

Viral metagenomics, also known as virome, is a new branch of science that emerged based on metagenomics theory, in combination with current virology and molecular biology techniques. Surpassing the limitations of conventional viral study approaches, virome directly targets the genetic material of all viruses present in the environment or tissue, enabling rapid and precise identification of viruses. It is powerful for uncovering novel viruses, studying virus population structure and diversity, and monitoring virus mutations and evolution. 
 

Future research trends

In recent years, many high-score articles on the macro virus group have been published. The research objects include soil, water and human body. This shows that the macro virus group has attracted the attention of scientific researchers from all walks of life, and as the target of follow-up research, it is called "small virus, everywhere; Virus group, great future!"

High score classic article

Articles

Journals

Year

The evolutionary history of vertebrateRNA viruses

Nature

2018

Marine DNA Viral Macro-and Microdiversity from Pole to Pole

Cell

2019

The stepwise assembly of the neonatal virome is modulated by breastfeeding

Nature

2020

Breastfeeding influences the neonatal virome

Nature

2021

Cryptic and abundant marine viruses at the evolutionary origins of Earth’s RNA virome

Science

2022

Wastewater sequencing reveals community and variant dynamics of the collective human virome

Nature Communications

2023

The number of articles in the macro virus group continues to rise, and unknown areas need to be explored by global researchers, as if the macro virus group has become a research hotspot in the future.

 

Technical parameters

Products

sequencing platform

sequencing strategy

Macro Virus Group

New PE150

10G raw data

Technical route

Viruses are ubiquitous, yet their enrichment remains challenging across various samples including human, animal, plant, marine, and soil due to their small genomes, low nucleic acid content, and susceptibility to host interference during processing. This poses a significant obstacle for the study of Viral metagenomics. Magigene has developed two efficient virus enrichment strategies to assist researchers in overcoming these challenges.

ultracentrifugation:Pre-filtered samples are subjected to sucrose density gradient ultracentrifugation for over 24 hours for selective virus particles collection.

VirOne Competitive Precipitation: VirOne® competitive precipitation kit was developed by Magigene, which competitively binds and neutralizes the surface charges of water molecules, forcing the VLPs component to precipitate from the solution, thereby the viral particles can be precipitated rapidly by bench top centrifuge.

Both approaches have been proven to be effective and reliable through extensive tests. For further information, please consult our local marketing and sales personnel. 

Technical pipeline
Tissue homogenization -- Membrane filtration -- Ultracentrifugation or VirOne® precipitation -- Nuclease treatment -- Virus nucleic acid extraction -- Library construction -- Sequencing and bioinformatics analysis.

Experimental process

tissue homogenate
membrane filtration
Ultracentrifugation or VirOne Precipitant
nuclease treatment
virus nucleic acid extraction
Library preparation and warehouse inspection
on-board sequencing

Analysis process

(1)DNA macro virus group analysis process

(1)RNA macro virus group analysis process

Case analysis

Case 1: Response of soil viral communities to land use changes-Response of soil virus community to land use change[1]
Journal:Nature communicationsTime: 2022.10.12 DOI:10.1038/s41467-022-33771-2
In this study, in order to explore the response of soil virus communities to changes in land use types during rapid urbanization, researchers collected five soil samples with different land uses and analyzed their virus and bacterial communities in depth, obtaining nearly 60,000 new soil virus populations, and found that land use changes affected the lifestyle of soil virus communities and changed the virus-bacterial host interaction model; at the same time, the change of land use leads to the differentiation of soil virus ecological niche, which drives the evolution of soil virus community.

 

Interrelationships among host bacteria, viruses, and the environment

Case2:Mesophilic and thermophilic viruses are associated with nutrient cycling during hyperthermophilic composting[2]
-- Mesophilic and thermophilic viruses associated with nutrient cycling during high-temperature composting
Journal:The ISME JournalTime: 2023.04.08 DOI:10.1038/s41396-023-01404-1
This paper investigates the importance of mesophilic and thermophilic bacteria and their viruses to nutrient cycling during industrial-scale hyperthermophilic composting (HTC). The results showed that the virus-bacteria density kinetics and activity were tightly coupled, and the virus unique to mesophilic bacteria encoded and expressed several carbon cycle-related accessory metabolic genes (AMG), which affected the nutrient turnover of bacteria. Most of the RNA viruses detected were associated with eukaryotes and not with nutrient cycling during the thermophilic phase of composting. Studies have shown that DNA viruses can drive nutrient cycling during HTC by recovering bacterial biomass through cell lysis and expression of key AMGs. Therefore, viruses may be used as indicators of microbial ecosystem function to optimize the productivity of biotechnology and agricultural systems.


 

Viral-host linkages between viral taxonomy (family level) and bacterial MAGs

References:

[1] Liao H, Li H, Duan C S, et al. Response of soil viral communities to land use changes[J].Nature Communications, 2022, 13(1): 6027.
[2] Liao H, Liu C, Ai C, et al. Mesophilic and thermophilic viruses are associated with nutrient cycling during hyperthermophilic composting[J].The ISME Journal, 2023: 1-15.
 

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