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Environmental DNA (eDNA) biodiversity detection

Environmental DNA (eDNA) biodiversity detection


Environmental DNA

Environmental DNA (edna) is DNA collected from vary environmental samples such as water, soil, and air. It is a mixture of DNA from different species such as microorganisms, animals, plants, etc. eDNA included DNA released into the environment by living organisms through skin, hair, scales, urine, feces, mucus, etc., and DNA released into the environment after death. So by using extracted eDNA,the specific gene fragments, usually located in mitochondrial genomes, were amplified by universal primers and the sequenced. The species were then identified by blast all the sequence against database. Finally, the biodiversity was obtained by integrated results.
 

Technology of Environmental DNA Macro Barcoding

Environmental DNA macro bar code technology is to extract the genetic information of organisms from environmental samples such as water, air and soil, amplify the species-specific sequences (located on chromosomes or mitochondrial genomes) in the common regions of different species by using appropriate primers, and then identify the species after high-throughput sequencing to obtain the DNA sequence to obtain the species community information in the environment.

  • Sample collection
  • DNA extraction
  • PCR amplification
  • High-throughput sequencing
  • Sequence filtration and blast
  • Biological information analysis

Technical features

Product Content

Sample Requirements

self-built database

Application of Environmental DNA Technology

  • Red list of China’s Biodiversity
  • IUCN Red List of Threatened Species
  • Invasive alien species

Environmental DNA Authoritative Standards and Specifications

Advantage of Yijian Environmental DNA Products

  • Comprehensive database
    Integrated databases with manual correction. 
    >7M reference sequences. 
  • Species list
    Different species lists were provided for search, such as IUCN Red List of Threatened Species. 
  • Personalized service
    Provide personalized database construction services, biological groups screening according to ecosystem or other requirements.

Yijian Environmental DNA Product Service Process

  • Pre-sales consultation 
  • Contract signing
  • Samples transportation
  • Experiment and analysis
  • Report integrattion

Classic Articles

Article one:

In this study, environmental DNA was used to analyze the diversity, community structure and co-occurrence pattern of biogeographic patterns of eukaryotic plankton in three coastal areas of Northeast, central and southern China. The results showed that the coastal marine plankton diversity showed a strong regional specific pattern, and the environmental factors inorganic nitrogen and heavy metals had the greatest influence on the plankton community similarity in Beibu Gulf (BB) and East China Sea (ECS). In addition, nutrients and HMs have a significant impact on the spatial symbiosis pattern and network topology of plankton.

References:
Zhang.S.Y Zheng,A. Zhan,CDong,J. Zhao & M, Yao (2022)Environmental DNA captures native and non-native fish community variations acro
thelentic andlotic systems ofa megacity. SciAdv,8, eabk0097.

 

Article two:
Environmental DNA macro-barcode sequencing of 109 site-and site-specific fish communities in Beijing identified 52 local and 23 non-local taxa, and found that fish diversity has little effect on urban land cover change, but the relative sequence abundance of non-native water bodies increases linearly with distance from the urban center. The results illustrate the complex impact of urbanization on local and non-native fish in different aquatic habitats, the importance of protecting urban aquatic biodiversity was emphasized.
 

References:
(2) Zhang,Z,J.Li,H.Li,L.Wang,Y.Zhou,S.Li,Z.Zhang,k. Feng & Y.Deng (2023) Environmental DNA metabarcoding reveals the influence of human
activities on microeukaryotic plankton along the Chinese coastline. Water Res.233.119730.

 

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