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Antibiotic resistance gene chip

Antibiotic resistance gene chip


Product Introduction

Based on high-throughput OPCR(HT-OPCR) quantitative detection technology, corresponding GPCR primers were designed for antibiotic resistance genes, and packaged into thin-layer metal alloy nanopore chips to obtain high-throughput aPCR chips. At the same time, based on the SmartChip Real-Time PCR Svstem, high-throughput automatic micro-sampling and gene quantitative analysis platform, 5.184 reactions and data analysis can be completed at a high speed at one time, and target gene detection and quantitative calculation with high efficiency, high throughput, high accuracy and high sensitivity can be carried out on multiple samples, thus avoiding the disadvantages of single gene detection, high cost and low efficiency of the previous traditional OPCR method.

High-throughput qPCR chip detection platform

Product Classification:

Antibiotic resistance gene chip for quantitative detection of antibiotic resistance genes and genes related to horizontal gene transfer, covers more than 10 antibiotic resistance gene classes including B- lactam (B-Lactamase), tetracyclines (Tetracycline), sulfonamides (Sulfonamide), glycopeptides (Glycopeptide), aminosugars (Aminoglycoside), diaminopyridines (Diaminopyrimidine), flunonones (Fluoroquinolone), multidrug resistance (Multidrug) and MLSB. At present, the antibiotic resistance gene chip includes 384P and 144P specifications. It can be widely applied to the accurate quantification of resistance gene markers in various environmental samples, such as water (lake, ocean, sewage, groundwater, etc.), soil, plants, sediments, feces, aerosol particles, etc.

chip functional gene distribution:

Detection of gene function distribution by antibiotic resistance gene chip (384p)

Detection of gene function distribution by antibiotic resistance gene chip (144p)

Application:

  • Abundance and distribution characteristics of resistance genes
  • Transmission and diffusion mechanism of resistance genes
  • Study on the management of antibiotic use
  • Relationship between resistance genes and environmental microorganisms

Product advantages

Experimental process:

Sample Collection
Nucleic acid extraction and quality inspection
qPCR chip reaction
Data processing

Analysis process

Analysis results display

Gene testing Wayne diagram

Sample correlation heatmap

gene absolute quantitative heat map

Gene family stacking map

Top10 gene histograms with the most significant differences in ANOVA analysis

 

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Web:www.magigene.com

Address: Floor 13, Block B, Building 7, Phase 3, International Innovation Valley, Shenzhen

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