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Magigene chip

Magigene chip


Product Introduction

Cyanobacteria is an important microorganism group in both freshwater and seawater and plays roles in carbon cycle and food chain. But blooms are global environmental problems which cause loss of health and economy. Based on the complete genome sequences of 1,322 cyanobacteria,we developed Cyanochip, which is a DNA array including 60K probes. It can achieve strain-level identification and quantitative detection of cyanobacteria from different environmental samples, such as water and soil. By two-color fluorescent labeling, one chip can examine 16 samples. Cyanochip can be applied to the monitor of cyanobacteria community changes of environmental samples and provide the support for early warning and forecasting of bloom outbreaks.

Product Application

Test case

Case 1: Detection of water samples in the early, middle and late stages of the cyanobacteria outbreak in Chaohu Lake
Results The repeatability was high: the difference in the group was significantly lower than the difference between the groups, and the detection rate of environmental factors associated with the reduction of community diversity and the strong growth of individual strains was higher than that of 16S.
 

Case 2: Detection of water samples from ten different sources across the country
The experimental samples were taken from 10 different places in China (Zhengzhou, Lanzhou, Xiamen, Shenzhen, Guangzhou, Xi'an, Kunming, Hefei, Beijing, Chongqing), a total of 32 samples were tested on 2 chips at one time. A total of 419 different types of cyanobacteria were detected, and an average of 144 cyanobacteria were detected in each sample, with the highest detection of 356 species and the lowest 50 species. NMDS analysis showed greater between-group differences and smaller within-group differences, indicating greater similarity in cyanobacterial community composition and structure between samples from the same source.
 

Cyanobacteria

Cyanobacteria also known as cyanobacteria, blue-green algae. Gram-negative, chloroplast-free (as distinguished from eukaryotes, algae), large unicellular prokaryotes capable of oxygenic photosynthesis, widespread in a variety of water bodies and other environments. In some nutrient-rich water bodies, some cyanobacteria often multiply in large numbers in summer, and form a layer of blue-green and fishy-smelling foam on the water surface, called "bloom".
The harm of cyanobacteria: affecting the environmental landscape, affecting the safety of water supply in water sources, destroying the aquatic ecosystem, and cyanobacterial toxins endangering the health of humans and other animals.
 

environmental function gene chip

The environmental functional gene chip represented by GeoChip is an important direction of the development of the international gene chip industry, and is one of the important means of global microecology and environmental genomics research, which is suitable for the efficient detection and quantification of specific genes, species, and functional processes in complex samples. High-throughput gene chip technology can greatly reduce the non-specific nucleic acid sequence interference, has the characteristics of high sensitivity and high reproducibility, and has a profound impact on the ecological environment monitoring. Since the successful development of the first generation chip GeoChip1.0 in 2002, there have been more than 1000 SCI papers based on GeoChip methods, resulting in a large number of theoretical and applied research results.

Cyanobacteria chip (CyanoChip)

Cyanobacteria is an important microorganism group in both freshwater and seawater and plays roles in carbon cycle and food chain. But blooms are global environmental problems which cause loss of health and economy. Based on the complete genome sequences of 1,322 cyanobacteria,we developed Cyanochip, which is a DNA array including 60K probes. It can achieve strain-level identification and quantitative detection of cyanobacteria from different environmental samples, such as water and soil. By two-color fluorescent labeling, one chip can examine 16 samples. Cyanochip can be applied to the monitor of cyanobacteria community changes of environmental samples and provide the support for early warning and forecasting of bloom outbreaks.

 

 

 

Cyanobacteria chip design

Experimental process

Sample Collection
DNA extraction
Nucleic Acid Detection
fluorescent marker
chip hybridization
chip scanning imaging

Analysis process

Product Delivery Results

Product advantages

  • Advantages
    Comprehensive database
    The target cyanobacteria of the current Cyanochip version include 1,322 strains which belong to 105 genera. It already all well-studied cyanobacteria genera such as Microcystis, Synechococcus, and Nostoc. With the new studies of cyanobacteria genomes, the list will be expanded continuously. The probes of important genes will be added in the following update.
  • High accuracy
    For each cyanobacteria strain, average ~40 probes significantly increase the accuracy of identification, compared to the traditional single fragment detection of PCR. The sensitivity of Cyanochip is 0.1ng nucleic acid for specific target species.
  • Short detection time
    Testing process < 2 days

Sample advantage

Filter Membrane Samplenucleic acid sample
The water sample shall be a filtered filter membrane with a membrane volume of 1-5L.DNA concentration ≥ 20ng/ul DNA amount> 500ng/sample
Sample preservation: placed in-80C refrigerator for long-term preservationSample DNA preservation: placed in-80C refrigerator for long-term preservation
Sample transportation: the sample is placed in the PE tube, the use of dry ice transportSample transportation: the sample is placed in the PE tube, the use of dry ice transport

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Thank you for your support and trust in Meg Gene.

Magi Gene

Magi Kefu

Contact Us

Web:www.magigene.com

Address: Floor 13, Block B, Building 7, Phase 3, International Innovation Valley, Shenzhen

Address: 28th Floor, Building A, Guanzhou Life Science Center, Guangzhou International BioIsland

Address: 4F, Building 3, Phase 2, Guangzhou International Biological Island

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