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High-throughput qPCR chip customization

Introduction

According to custom’s requirements, we provide design and optimization of a high-throughput qPCR chip. It is based on Wafegen^® SmartChip Real-Time PCR System and used for multiple genes quantification. An integrated chip can perform 5,184 qPCR reactions at the same time.

Experimental process

The SmartChip MultiSample NanoDispenser platform prepares nano-scale gPCR reaction systems
The SmartChip Real-Time PCR Cycler completes the qPCR reaction and obtains the reaction Ct value, melting curve and amplification curve

Representative products and articles

Representative product

Antibiotic resistance gene chip
Carbon, nitrogen, phosphorus, sulfur functional gene chip
Arsenic functional gene chip
Heavy metal resistance gene chip
Virulence factor gene chip
Environmental fecal pollution traceability chip
Pathogenic bacteria quantitative chip

Representative article

Article title	Journal
MRG Chip: A High-Throughput qPCR-Based Tool for Assessment of the Heavy Metal (loid) Resistome	Environmental Science & Technology
VFG-Chip: A high-throughput qPCR microarray for profiling virulence factor genes from the environment	Environment International
Diverse and abundant antibiotic resistance genes in Chinese swine farms	PNAS
QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling	Science China Life Sciences
AsChip: A High-Throughput qPCR Chip for Comprehensive Profiling of Genes Linked to Microbial Cycling of Arsenic	Environmental Science & Technology
High-throughput diagnosis of human pathogens and fecal contamination in marine recreational water	Environmental Research

Advantages

High throughput
Hundreds of target genes can be detected simultaneously.
Low usage
The volume of each reaction system is low to 100 nanoliter to greatly reduce the cost of the reagents.
Cost-effective
Low cost for single gene quantification compared to traditional microliter-scale PCR systems.
Absolute quantification
Based on the absolute quantification of the 16S rRNA, the absolute quantification of all other genes can be calculated.

Customized delivery

List of primers
Information such as the sequence of the primers, the length of the amplified fragments, the gene names, and functions, etc.
Explanation of experimental operation and data analysis
Operator's manual and training for the experimental process including sample requirements, quality control requirements, amplification curve, melting curve interpretation, Ct value interpretation standards, quantitative calculation methods, etc.
R&D process report
Primer design, experimental validation, and other related record of R&D.

Service process

Custom provides the target microbial species or genes
We provide quotations and sign service contract
High-throughput qPCR chip design
High-throughput qPCR chip validation and optimization
Customized service delivery

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Information to be filled in when ordering products:

In order to provide you with faster and better service, please fill in the following information carefully. After receiving the inquiry form, we will evaluate your information, contact you and give you a quotation.

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