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Eukaryotic RNA Sequencing

Product Introduction

The eukaryotic RNA Sequencing is mainly divided into two categories based on the availability of a reference genome: eukaryotic RNA sequencing with reference and eukaryotic RNA sequencing without reference.
Eukaryotic RNA sequencing with reference refers to the transcriptome sequencing of eukaryotic organisms, their tissues, or cells that already have a reference genome. By aligning the sequencing results with the reference genome, one can verify the sequencing saturation and gene coverage of the RNA sequencing. Additionally, information such as gene structure, gene expression differences, and alternative splicing can be obtained.
Eukaryotic RNA sequencing without reference refers to the sequencing of eukaryotic RNA transcriptomes in the absence of a reference genome. During eukaryotic RNA sequencing without reference, after obtaining the raw data, quality control and assembly into uni-gene are first required. Subsequent analyses are then conducted using these uni-genes as the reference sequences. Differential gene expression analysis and functional enrichment analysis of differential genes can also be performed on multiple samples to discover functional genes and provide directions for further research.

Product Features

High coverage: A single transcriptome sequencing experiment can provide comprehensive information on coding RNAs.
Strand-specific libraries: Uses a dUTP strand-specific library preparation method to ensure the directionality of transcripts, leading to more accurate quantification results.
Integrated analysis: Can be combined with multiple omics datasets for cross-omics association analysis.

Technical Parameter

Product	Sequencing Platform	Data Volume
Eukaryotic RNA Sequencing	Nova PE150	6 G raw data

Experimental Process

Analysis Process

（1）Eukaryotic RNA Sequencing With Reference

（2）Eukaryotic RNA Sequencing Without Reference

Presentation of Analysis Results

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