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High-Throughput qPCR Chip

Product Introduction

High-throughput qPCR (HT-qPCR) chip quantitative detection technology is designed for the target functional genes, corresponding qPCR primers are designed and packaged into a thin-layer metal alloy nanopore chip to obtain a high-throughput qPCR chip. At the same time, based on the SmartChip Real-Time PCR System, high-throughput automatic micro-dosing and gene quantitative analysis platform, it can complete 5184 reactions and data analysis at one time, and can carry out efficient, high-throughput, highly accurate and highly sensitive target gene detection and quantitative calculations for multiple samples, avoiding the singleness of gene detection, high cost and low efficiency of traditional qPCR methods.

High-throughput qPCR chip detection platform

High-throughput qPCR chip detection process

Product List

High-throughput qPCR chip product list

Product	Number Of Gene Primers Detected (Including 16S Gene)	Number of Samples Tested on a Single Chip (Including 3 Technical Duplicates)	Application
Antibiotic Resistance Gene Chip(384P)	384	4	(1) Abundance and distribution characteristics of resistance genes (2) Mechanism of resistance gene transmission and diffusion (3) Research on the management of antibiotic use (4) Relationship between resistance genes and environmental microorganisms
Antibiotic Resistance Gene Chip(144P)	144	12
Quantitative Microbial Element Cycling Gene Chip	72	24	(1) Study on the geochemical cycle of carbon, nitrogen, phosphorus, and sulfur (2) Microbiological mechanisms of carbon, nitrogen, phosphorus, and sulfur conversion (3) Formulation of fertilization management measures for farmland (4) Response of ecosystem functions to climate change
Arsenic Functional Gene Chip	80	21	(1) Research on microbial arsenic transformation and metabolism (2) Microbial arsenic cycle (3) Investigation and management of environmental arsenic pollution (4) Arsenic pollution treatment
Heavy Metal Resistance Gene Chip	96	18	(1) Study on microbial mediated metal biochemical processes (2) Detection and characterization of environmental metal resistance genes (3) Investigation and management of heavy metal pollution
Virulence Factor Gene Chip	120	14	(1) Research on the pathogenic mechanism and potential of environmental pathogens (2) Health risk assessment of zoonotic pathogens (3) Research on the mechanism of drug action

Technical Characteristics

High Detection Throughput
High Specificity
Low Sample Usage
Absolute Quantification
Cost-Effective

Comparison of high-throughput qPCR chip with metagenome

Comparison Point	High Throughput qPCR Chip	Metagenome
Objective	Study on the composition and abundance of functional genes in the sample population
Detect The Number of Genes	Dozens to hundreds	Over ten thousand
Detection Period	Short	Long
Sensitivity	High	Low abundance genes are difficult to detect
Host Interference Level	low	High
Gene Quantification	Relative, Absolute	Relative
Sample Dosage	Low	High
Data Utilization	Only genes in the list can be analyzed	Able to explore new species and genes

Experimental Process

Analysis process

Project Articles

Articles 1：Effect of phenol formaldehyde-associated microplastics on soil microbial community, assembly, and functioning

Journal: Journal of Hazardous Materials

Publication Date: 2022-10-31

This study investigates the impact of phenol formaldehyde-associated microplastics (PF-MPs) on soil microbial and micro-eukaryotic communities, as well as their functions, revealing that high concentrations (1%) of PF-MPs reduce microbial interactions and increase the role of stochastic processes in community assembly, altering bacterial but not eukaryotic communities. The research also identifies a negative correlation between N₂O emissions and nitrification-related functional genes, indicating impacts on nitrogen cycling. Furthermore, it shows that higher PF-MPs concentrations are associated with a slight decrease in vegetable biomass, which is positively correlated with the diversity of micro-eukaryotes and the functional diversity of bacteria. These findings highlight the potential for PF-MPs to affect crop growth by disrupting microbial community dynamics and diversity, emphasizing the need for careful agricultural management and consideration of the risks posed by these pollutants to soil ecosystems.

Functional genes with difference in the absolute abundance between the soils amended with different concentrations of PF-MPs.

Articles 2：Effects of heavy metal and disinfectant on antibiotic resistance genes and virulence factor genes in the plastisphere from diverse soil ecosystems

Journal: Journal of Hazardous Materials

Publication Date: 2023-12-21

This study investigates the impact of combined exposure to quaternary ammonium compounds (QACs) and copper (Cu) on the abundance and profile of antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) in the plastispheres of three different soil types. The research reveals that plastispheres not only enrich ARGs compared to bulk soil but also enhance the coupled effect of these pollutants, promoting the proliferation of ARGs and VFGs, likely through horizontal gene transfer. Furthermore, the study finds that the relative abundances of potential pathogens increase under the combined stress, with the effects varying by soil type, underscoring the importance of considering soil characteristics in health risk assessments. The findings highlight the elevated health risks associated with ARGs in plastispheres subjected to multiple selective pressures and contribute valuable data for future antibiotic resistance risk management.

Network analyses displaying co-occurrence patterns between ARGs, MGEs, VFGs and microbial taxa at the genus level in (a, b, c) soils and (d, e, f) plastispheres

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