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Relative Quantitative 16S/18S/ITS Amplicon Sequencing

Product Introduction

Amplification sequencing refers to using suitable universal primers to amplify the 16S rDNA/18S rDNA/ITS high-variable region or functional genes in microorganisms under environmental conditions, and analyzing the diversity and distribution rules of microbial communities in the environment by high-throughput sequencing technology to detect sequence variation and abundance information of PCR products. This can reveal the types and relative abundances of many microorganisms in environmental samples and their evolutionary relationships.
Based on the characteristics of sequencing technology platforms, amplicon sequencing methods can primarily be divided into two major categories: Short-Read Sequencing and Long-Read Sequencing. Each of these approaches has ITS own advantages and disadvantages and is suitable for different research needs.

Technical Parameter

Product	Sequencing Platform	Data Volume
16S/18S/ITS/Functional Genes High-Throughput Sequencing	NovaSeq PE250/MiSeq PE300	60 K/30 K raw reads
Long-Read Sequencing Amplicon Sequencing	PacBio sequel II	30 K CCS raw reads

Experimental process

Analysis process

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