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Cyanochip

Introduction

Cyanobacteria is an important microorganism group in both freshwater and seawater and plays roles in carbon cycle and food chain. But blooms are global environmental problems which cause loss of health and economy. Based on the complete genome sequences of 1,322 cyanobacteria，we developed Cyanochip， which is a DNA array including 60K probes. It can achieve strain-level identification and quantitative detection of cyanobacteria from different environmental samples, such as water and soil. By two-color fluorescent labeling, one chip can examine 16 samples. Cyanochip can be applied to the monitor of cyanobacteria community changes of environmental samples and provide the support for early warning and forecasting of bloom outbreaks.

Applications

Monitor the diversity and succession rules of cyanobacteria in environmental samples
Study the relationships between cyanobacteria and environmental factors
Study the roles of cyanobacteria in carbon cycle
Study the symbiosis of cyanobacteria and the host moss
Forecast the bloom outbreaks

Advantages

Comprehensive database
The target cyanobacteria of the current Cyanochip version include 1,322 strains which belong to 105 genera. It already all well-studied cyanobacteria genera such as Microcystis, Synechococcus, and Nostoc. With the new studies of cyanobacteria genomes, the list will be expanded continuously. The probes of important genes will be added in the following update.
High accuracy
For each cyanobacteria strain, average ~40 probes significantly increase the accuracy of identification, compared to the traditional single fragment detection of PCR. The sensitivity of Cyanochip is 0.1ng nucleic acid for specific target species.
Short detection time
Testing process < 2 days

Experiment process

Sample collection
DNA extraction
Fluorescent labeling
Chip hybridization
Chip scanning
Data analysis
Report preparation

Sample requirements

Sample type	Sample requirements
Membrane	For each sample, 1-5 L of water was filtered using the membrane of 0.22um/0.45um. The membranes were stored at -80°C. Please place filter membranes in PE tubes and use dry ice for transportation.
Nucleic acid	DNA concentration：≥20ng/ul DNA total amount：＞500ng/sample

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