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Introduction to Sequencing Platform

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No reference genome species can do BSA trait mapping?

Species without reference genomes can be analyzed for SNP frequency differences, and candidate segments can be found, but the effect of non-reference positioning is poor, and the lack of annotation files, and the results obtained are few. In general, BSA trait mapping is not recommend for non-ginseng species, and it is recommended to try genetic mapping and other schemes.

Can the size of the candidate region and the number of candidate genes be guaranteed?

The size of the candidate region and the number of candidate genes are related to the size of the population, the size of the difference in parental material, the characteristics of the target trait, the depth of sequencing, the level of the genome of the analyzed species and many other factors, so a unified standard cannot be given, but it can be estimated with reference to the experience of the completed project.

How to mix DNA samples? Mix samples first and then extract DNA? Or do you want to extract DNA first and then mix the same amount?

The systematic error can be reduced by first mentioning DNA and then mixing it in equal amounts. Most of the literatures published in recent years are first mentioning DNA and then mixing it in equal amounts.

Simplified genome BSA is cheaper than whole genome BSA?

Simplify genome technology, capture genome information accounts for about 1% to 10% of the genome size, whole genome technology, sequencing the entire genome, obtaining more comprehensive variation information, higher cost performance, and the unit price of data is at least 25 times cheaper.

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